DTT dithiothreitol cas:3483-12-3 purification method

High purity of DTT synthesized by current methods or commercially available.

And the two immiscible solvents are prepared into a two-phase solution without insoluble solids under heating or normal temperature conditions, and then the two-phase system is cooled to precipitate crystals. One of the two insoluble solvents is water, and the other is an organic solvent insoluble in water.

1, 2-dichloroethane, methyl acetate, ethyl acetate, propyl acetate, butyl acetate, n-butanol, isobutanol, SEC butanol, tert butanol, alkanes containing 4-10 carbon atoms, petroleum ether, benzene, benzene containing substituents, and butanone.

The invention provides a method for improving the quality of DTT through recrystallization, which is simple to operate and easy to industrialize. The method provided by the invention is used to recrystallize DTT, and the obtained DTT product has better appearance and purity than that obtained by recrystallizing DTT with single-phase solvent system, so it has higher added value. DTT is recrystallized. Due to the existence of oil-water interface in the crystallization system, the nucleation mechanism is different from that under general crystallization conditions, and the crystal form and purity are also different. This is the theoretical basis for the method provided by the invention to provide excellent DTT products.

DTT is purified by recrystallization. By controlling crystallization parameters, crystals with different crystal forms and purity can be obtained. The initial crystal nucleus has an important influence on the crystal form and purity of the obtained crystal